uncoupling agent carbonyl cyanide p Search Results


carbonyl cyanide 4 trifluomethoxy phenylhydrazone  (Tocris)
95
Tocris carbonyl cyanide 4 trifluomethoxy phenylhydrazone
Carbonyl Cyanide 4 Trifluomethoxy Phenylhydrazone, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/carbonyl cyanide 4 trifluomethoxy phenylhydrazone/product/Tocris
Average 95 stars, based on 1 article reviews
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oxphos  (MedChemExpress)
96
MedChemExpress oxphos
Oxphos, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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fccp  (Agilent technologies)
90
Agilent technologies fccp
Fccp, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90/100 stars
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fccp (mmp uncoupler)  (Millipore)
90
Millipore fccp (mmp uncoupler)
GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. <t>FCCP</t> (uncoupler cause mitochondrial membrane <t>potential</t> <t>(MMP)</t> dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
Fccp (Mmp Uncoupler), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fccp (mmp uncoupler)/product/Millipore
Average 90 stars, based on 1 article reviews
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1μm carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (fccp) (mitochondrial uncoupler)  (Millipore)
90
Millipore 1μm carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (fccp) (mitochondrial uncoupler)
GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. <t>FCCP</t> (uncoupler cause mitochondrial membrane <t>potential</t> <t>(MMP)</t> dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
1μm Carbonyl Cyanide P Trifluoromethoxyphenylhydrazone (Fccp) (Mitochondrial Uncoupler), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1μm carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (fccp) (mitochondrial uncoupler)/product/Millipore
Average 90 stars, based on 1 article reviews
1μm carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (fccp) (mitochondrial uncoupler) - by Bioz Stars, 2026-03
90/100 stars
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fccp (uncoupling agent)  (Merck KGaA)
90
Merck KGaA fccp (uncoupling agent)
GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. <t>FCCP</t> (uncoupler cause mitochondrial membrane <t>potential</t> <t>(MMP)</t> dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
Fccp (Uncoupling Agent), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fccp (uncoupling agent)/product/Merck KGaA
Average 90 stars, based on 1 article reviews
fccp (uncoupling agent) - by Bioz Stars, 2026-03
90/100 stars
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fccp (uncoupling agent, 0.5 μm)  (Agilent technologies)
90
Agilent technologies fccp (uncoupling agent, 0.5 μm)
GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. <t>FCCP</t> (uncoupler cause mitochondrial membrane <t>potential</t> <t>(MMP)</t> dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
Fccp (Uncoupling Agent, 0.5 μm), supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fccp (uncoupling agent, 0.5 μm)/product/Agilent technologies
Average 90 stars, based on 1 article reviews
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90/100 stars
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fccp  (Millipore)
90
Millipore fccp
GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. <t>FCCP</t> (uncoupler cause mitochondrial membrane <t>potential</t> <t>(MMP)</t> dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
Fccp, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fccp/product/Millipore
Average 90 stars, based on 1 article reviews
fccp - by Bioz Stars, 2026-03
90/100 stars
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fccp  (ApexBio)
90
ApexBio fccp
GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. <t>FCCP</t> (uncoupler cause mitochondrial membrane <t>potential</t> <t>(MMP)</t> dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
Fccp, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fccp/product/ApexBio
Average 90 stars, based on 1 article reviews
fccp - by Bioz Stars, 2026-03
90/100 stars
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tri uoromethoxy carbonylcyanide phenylhydrazone (fccp; oxidative phosphorylation uncoupler  (Millipore)
90
Millipore tri uoromethoxy carbonylcyanide phenylhydrazone (fccp; oxidative phosphorylation uncoupler
GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. <t>FCCP</t> (uncoupler cause mitochondrial membrane <t>potential</t> <t>(MMP)</t> dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
Tri Uoromethoxy Carbonylcyanide Phenylhydrazone (Fccp; Oxidative Phosphorylation Uncoupler, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tri uoromethoxy carbonylcyanide phenylhydrazone (fccp; oxidative phosphorylation uncoupler/product/Millipore
Average 90 stars, based on 1 article reviews
tri uoromethoxy carbonylcyanide phenylhydrazone (fccp; oxidative phosphorylation uncoupler - by Bioz Stars, 2026-03
90/100 stars
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trifluorocarbonylcyanide phenylhydrazone (fccp) (mitochondrial uncoupler) (5 lm)  (Agilent technologies)
90
Agilent technologies trifluorocarbonylcyanide phenylhydrazone (fccp) (mitochondrial uncoupler) (5 lm)
GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. <t>FCCP</t> (uncoupler cause mitochondrial membrane <t>potential</t> <t>(MMP)</t> dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
Trifluorocarbonylcyanide Phenylhydrazone (Fccp) (Mitochondrial Uncoupler) (5 Lm), supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trifluorocarbonylcyanide phenylhydrazone (fccp) (mitochondrial uncoupler) (5 lm)/product/Agilent technologies
Average 90 stars, based on 1 article reviews
trifluorocarbonylcyanide phenylhydrazone (fccp) (mitochondrial uncoupler) (5 lm) - by Bioz Stars, 2026-03
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5 mm mitochondrial uncoupler carbonyl cyanide p-trifluoromethoxy phenylhydrazone  (Millipore)
90
Millipore 5 mm mitochondrial uncoupler carbonyl cyanide p-trifluoromethoxy phenylhydrazone
GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. <t>FCCP</t> (uncoupler cause mitochondrial membrane <t>potential</t> <t>(MMP)</t> dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
5 Mm Mitochondrial Uncoupler Carbonyl Cyanide P Trifluoromethoxy Phenylhydrazone, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5 mm mitochondrial uncoupler carbonyl cyanide p-trifluoromethoxy phenylhydrazone/product/Millipore
Average 90 stars, based on 1 article reviews
5 mm mitochondrial uncoupler carbonyl cyanide p-trifluoromethoxy phenylhydrazone - by Bioz Stars, 2026-03
90/100 stars
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GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. FCCP (uncoupler cause mitochondrial membrane potential (MMP) dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

Journal: Scientific Reports

Article Title: Gasdermin D restricts Burkholderia cenocepacia infection in vitro and in vivo

doi: 10.1038/s41598-020-79201-5

Figure Lengend Snippet: GSDMD contributes to bactericidal mROS production during B. cenocepacia infection. (A) Macrophage associated CFU of B. cenocepacia ( B.c.) (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages in presence and absence of N-acetylcysteine (NAC) (3 mM). Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. (B) Mitosox assay in WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia ( B.c. ) (MOI10) at 6 h post-infection in absence or presence of glycine (5 mM). Data represent mean ± SEM (n = 8). Statistical analysis was performed using two-way ANOVA. ( C ) Fold change comparing the addition of Mitotempo (MT) (20 µM) treatment in macrophage associated CFU of B. cenocepacia (MOI10) in WT, gsdmd −/− , and casp11 −/− macrophages at 6 h infection. Data represent mean ± SEM (n = 10). Statistical analysis was performed using two-way ANOVA. (D) TMRM assay in WT and gsdmd −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. FCCP (uncoupler cause mitochondrial membrane potential (MMP) dissipation) (10 µM) was included as negative control. Fluorescence values were normalized to cell number as measured per well before normalization to the fluorescence of the non-infected wells. Data represent mean ± SEM (n = 12). Statistical analysis was performed using two-way ANOVA. ( E ) Cytokine release from WT, gsdmd −/− , and casp11 −/− macrophages treated as in ( C ). Data represent mean ± SEM (n = 11). Statistical analysis was performed using paired one-tailed student’s t-test. (F) Immunoblot analysis of GSDMD in isolated phagosomes from WT, gsdmd −/− , and casp11 −/− macrophages infected with B. cenocepacia (MOI10) at 6 h post-infection. (G) CFU of B. cenocepacia harvested from WT and gsdmd −/− macrophages and treated with H 2 O 2 for 30 min before plating. Data represent mean ± SEM (n = 3). Statistical analysis was performed using two-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

Article Snippet: As a positive control, cells were treated with 10 μM of FCCP (MMP uncoupler) (Millipore Sigma, C2759).

Techniques: Infection, Mitosox Assay, Membrane, Negative Control, Fluorescence, One-tailed Test, Western Blot, Isolation